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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Acquisition of genes encoding aminoglycoside-modifying enzymes.
B) Production of beta-lactamase enzymes.
C) Alteration of penicillin-binding proteins (PBPs).
D) Increased expression of efflux pumps.

2. An elderly patient in a long-term care facility develops a localized outbreak of painful vesicles along a dermatome. Tzanck smear of a vesicle reveals multinucleated giant cells. The MOST likelycausative agent is:

A) Varicella-zoster virus
B) Coxsackievirus
C) Herpes simplex virus type 1
D) Orf virus

3. A clinical microbiology laboratory is implementing a new MALDI-TOF mass spectrometry system for bacterial identification. For accurate identification, the system relies on comparing the protein profile of an unknown isolate to:

A) A database of protein mass spectra from well-characterized organisms.
B) A database of bacterial cell wall lipid compositions.
C) A database of antibiotic resistance genes.
D) A database of known bacterial ribosomal RNA sequences.

4. A 55-year-old male with a history of poorly controlled diabetes mellitus develops a necrotizing fasciitis of his left lower extremity following a minor abrasion sustained while gardening. Initialcultures of the wound reveal a polymicrobial flora, including Gram-positive cocci in chains and Gram-negative bacilli. Over the next 48 hours, despite broad-spectrum antibiotic therapy, the patient's condition deteriorates, and new bullae with hemorrhagic fluid appear. Gram stain of this fluid now predominantly shows large, Gram-positive bacilli with subterminal spores. Which of the following virulence factors is MOST likely contributing to the rapid tissue destruction observed in this patient?

A) Protein A
B) Lipopolysaccharide (LPS)
C) Exotoxin B (SpeB)
D) Lecithinase (alpha-toxin)

5. An immunocompromised patient develops a disseminated fungal infection. Blood cultures are consistently negative. However, a serum beta-D-glucan assay is markedly elevated. Tissue biopsy reveals the presence of hyaline, septate hyphae with dichotomous branching. The MOST likely causative organism is:

A) Aspergillus fumigatus
B) Rhizopus species
C) Mucor species
D) Cryptococcus neoformans

Solutions: